Establishment of A Mouse Model of Stellate Ganglion Block and Subsequent Biphasic Effects on Bilateral Cerebral Cortical Blood Flow

Authors

  • Jiahua Wang Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Wei Zhou Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Xiaohong Wang Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Shiting Yan School of Medicine, Yangzhou University, Yangzhou, China;
  • Shunping Yan School of Medicine, Yangzhou University, Yangzhou, China
  • Ying Wang Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Yanlong Yu Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Hu Li Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Dongsheng Zhang Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;
  • Zhuan Zhang Affifiliated Hospital of Yangzhou University, Yangzhou University, Yangzhou, China;

DOI:

https://doi.org/10.31636/pmjua.v8i1-2.5

Keywords:

Stellate ganglion block, Mouse model, Cerebral blood flow, Sympathetic nerve system

Abstract

Background and objectives: Stellate ganglion block (SGB) has significant therapeutic efficacy in various clinical practices and further exploration of SGB is needed. The mouse model of SGB had not been reported and the effect on cerebral cortical blood flow (CCBF) was controversial. We aimed to establish an stellate ganglion block (SGB) mouse model and explore how SGB influences CCBF.

Methods: Male C57BL/6 mice were randomly divided into five groups (n = 6): groups L (left SGB) and R (right SGB) received an injection of 0.25% ropivacaine hydrochloride (0.08 mL) on the respective stellate ganglion; similarly, groups SL (left saline control) and SR (right saline control) received an injection of normal saline (0.08 mL) instead. Group C received no intervention. CCBF was assessed before SGB (T0) and 10 (T1), 30 (T2), 60 (T3), 90 (T4), and 120 min (T5) after SGB using laser speckle contrast imaging system.

Results: The SGB mouse model was successfully established in groups L and R. Compared with baseline, CCBF on the blocked side decreased at T1, increased at T2–T5, and peaked at T3 in groups L and R (all, P < 0.01). Compared with groups C and SL, the CCBF on the left side decreased at T1 and increased at T2–T5 in group L (all, P < 0.05). A similar trend was noted in groups C and SR relative to group R. The CCBF on the unblocked side decreased at T1, increased at T2–T5, and peaked at T3 in groups L and R (all, P < 0.01).

Conclusions: The SGB mouse model was established successfully. Unilateral SGB can affect bilateral cerebral cortical blood flow, which shows a transitory decrease followed by a significant increase for at least 2 h.

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Establishment of a mouse model of stellate ganglion block and subsequent biphasic effects on bilateral cerebral cortical blood flow

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Published

2023-10-04

How to Cite

1.
Wang J, Zhou W, Wang X, Yan S, Yan S, Wang Y, et al. Establishment of A Mouse Model of Stellate Ganglion Block and Subsequent Biphasic Effects on Bilateral Cerebral Cortical Blood Flow. PMJUA [Internet]. 2023 Oct. 4 [cited 2026 Jun. 7];8(1-2):32-41. Available from: https://painmedicine.org.ua/index.php/pnmdcn/article/view/283

Issue

Vol. 8 No. 1-2 (2023): Pain Medicine

Section

Original article

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Copyright (c) 2023 Jiahua Wang, Wei Zhou, Xiaohong Wang, Shiting Yan, Shunping Yan, Ying Wang, Yanlong Yu, Hu Li, Dongsheng Zhang, Zhuan Zhang

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License

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